Boosting regeneration

Boosting regeneration


WR-cap TU




Landbouw, Water, Voedsel>Sleuteltechnologieën LWV>Biotechnologie en Veredeling






Many modern biotechnological tools in plant breeding such as interspecific hybridization, microsporogenesis, ploidy alterations, genetic modification and genome editing, rely on the ability to produce an entire plant from a single, improved cell. Hence, regeneration is key to a broad application of these technologies in a wide range of crops and for a wide range of goals, not in the least for circular agriculture.
This project tackles regeneration in a multi-faceted approach comprised in two work packages, using biochemistry and molecular genetics as instruments.
The research is performed by the academic partners WPR-Bioscience (WPR), WU-Organic Chemistry (ORC) and WUR-Plant Breeding (PBR), in close collaboration with 11 private partners.

Doel van het project

The goal of this project is to provide breeding companies with the generic tools to induce the regeneration capacity in their recalcitrant crops during the breeding process in a reversible and reproducible manner, to speed-up the breeding process, and as such the introduction of beneficial traits and novel sustainable crops.

Relatie met missie (Motivatie)

To make the transition to circular agriculture, it is essential to improve the resilience of crops to abiotic stresses, to produce more robust cultivars capable of dealing with different pests and diseases, to increase production with less chemical input, to facilitate a more sustainable glasshouse horticulture and lastly, to allow the secure production of safe foods.
To realize this ambition, the deployment of new plant breeding techniques, especially genome editing is an absolute requirement. The major bottle-neck to the successful application of these techniques is the possibility to regenerate entire plants from single edited of mutated plant cells. Also for other plant breeding expedients, e.g. anther culture, microspore development, embryo rescue, cell fusion and micropropagation, the process of regeneration is key.

Geplande acties

The intended results for 2020:
1. Following an inventory of peptides that have been used in the literature, we will identify a subset of peptides that may have the ability induce or enhance regeneration capacity. Start synthesizing peptides and test for their ability to induce regeneration.
2. Optimize a protoplast-to-plant system for testing peptides
3. Companies are to start setting up their own system, first using phytosulfokine (PSK), then using other positive peptides from the screen.
4. Clone a subset of regeneration candidate genes, start making constructs and set up a transient system to test constructs.
5. The project meetings are to be held twice per year to discuss the progress and agree on the next steps.

Naam projectleider

Frans Krens