Dietary fats (gebaseerd op AF-18077)
Recently it has become more and more evident that not so much the amount of fat, but rather the types of fat are important within the context of a healthy dietary pattern. Next to the more generally consumed long chain triglycerides (LCT, >C12:0), there are also ‘new fats’ on the market with their own properties and potential health benefits, such as phospholipids (PL) and fats containing medium chain triglycerides (MCT, C6:0-C12:0). These ‘new fats’ are most likely transported in the body in a different manner and can have a distinct effect on the uptake of vitamins, minerals and other compound compared to LCT. Therefore these ‘new fats’ most likely have a distinct effect on human health.
The primary objective of this project is to deliver novel, scientific insights on the nutritional and health potential of different types of lipid structures, with a special focus on:
1) New, alternative lipid structures and the impact on (DHA, C22:6) uptake and translocation
2) Modulating effects of different lipid structures on vitamin, mineral, choline and lutein uptake
A second objective of the project is to develop an in vitro screening method tailored towards lipid digestion, absorption and effects on uptake/transport/secretion of fat and health-related molecules. In the future this new innovative method can be used for fast, evidence based, screening of new fats, oils and modulating compounds on potential health effects related to dietary lipids.
Beoogde resultaten uit het projectplan:
1. Development of the in vitro screening tool box.
The models developed will focus on multiple nutritional and health-related levels, namely:
• Fat digestibility (based on INFOGEST protocol)
• Intestinal handling of the fats (e.g. lipid transport, chylomicron synthesis, bioavailability), using the Caco-2 intestinal cell model
• Fat-induced co-transport of health-related compounds from the intestinal lumen into the body (e.g. vitamins, minerals, lutein, choline), using the Caco-2 intestinal cell model
2. Using the in vitro screening tool box (see 1) to screen for nutritional and health-related effects of various lipid structures.
Using this tool box, lipid structures that were provided by the company (e.g. phospholipids and MCT) are screened for:
• Effect of digested lipids on intestinal integrity
• Bioavailability of digested lipids (chylomicron synthesis and transport of lipids, mainly DHA and MCT)
• (Co)translocation of vitamin/mineral, lutein, choline.
1. The in vitro screening tool box was developed and optimized:
• To study digestibility of the lipid samples, the INFOGEST protocol using the pH-STAT method that was originally set up for protein digestion, was in the current project optimized for fat digestion. For the digestion of lipids alone, this protocol optimisation seemed to be sufficient. For lipids combined with vitamins, minerals, choline and lutein, the adapted INFOGEST protocol was not very accurate. Especially lutein seemed to interfere. We alternatively used manual digestion protocols to be able to study lipid-induced co-translocation of vitamins, minerals and choline. It was collectively decided to eliminate lutein from the protocol for now, as solving this issue was not possible within the current project.
• The Caco-2 cell model was successfully optimised to study the effect of digested lipids on intestinal integrity, chylomicron synthesis, bioavailability of digested lipids (mainly DHA and MCT) and co-translocation of health-related compounds like vitamins, minerals and choline.
2. The lipid samples that were provided by the company were screened using the developed in vitro screening tool box (see result 1):
• For all lipid samples the digestibility was assessed using either the optimized INFOGEST protocol or the manual digestion protocol.
• All digested lipid samples were incubated with Caco-2 cells for 24 hours:
- The effect on intestinal integrity and chylomicron synthesis was assessed.
- The analyses for transported lipids (DHA, MCT) and co-translocation of vitamins, minerals and choline are outsourced to external parties outside WFBR. The results are expected in March/April 2020.
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