Plant breeding for protein processing efficiency and functionality from agricultural side-streams

Plant breeding for protein processing efficiency and functionality from agricultural side-streams


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Landbouw, Water, Voedsel>A. Kringlooplandbouw>A4. Eiwitvoorziening voor humane consumptie uit (nieuwe) plantaardige bronnen






Many high volume protein-rich side streams are not yet being used. For example, sugar beet leaves contain proteins with unique functional properties for food. So far, breeding, cultivation, and further processing of sugar beets have been optimized for carbohydrate yield. This project brings together supply chain partners to optimize sugar beets and other crops for protein production, to increase yield, extraction efficiency, and the business case of proteins from agricultural residues like leaves.

Doel van het project

This project contributes mostly to the following missions and key technologies:
• A3: Reuse of organic side and waste streams. The aim of this MMIP is to use organic waste and side streams as raw material for food (primarily) and non-food applications by 2030. This Project fits very well in this mission as we aim to use agricultural side or waste streams for the extraction of proteins primarily for food applications .
• A4: Protein supply for human consumption from (new) plant sources. The aim of this MMIP is to become less dependent on protein raw materials for (human) food and animal feed from outside Europe. We use crops grown here in the Netherlands such as sugar beet to make new protein ingredients. Due to the application of new technologies, these proteins will also have different functionalities than the proteins from current sources.
• ST2: Biotechnology and Breeding. One of the goals here is to enable breeding to combine and predict properties in such a way that breeding can respond more quickly to desired changes in production systems, for example suitable under Circular agriculture. The creation of more knowledge in the field about process and products specification will allows a faster and more effective crop breeding.

Relatie met missie (Motivatie)

This project connects to priority A8 “Verwerking van nieuwe kansrijke eiwitbronnen en of gewassen met voldoende volume die momenteel niet gebruikt worden tot hoogwaardige producten” by making proteins available from agricultural side streams from high volume crops

Geplande acties

Deliverables and Milestones:
D1.1: Report or presentation on characterization of proteins in leaves including their role in plant metabolism (M14)
D1.2: Report on the identification of potential bottlenecks for protein extraction in plants, either physical or chemical (M10, M40)
D1.3: Note on the conceptual design for use of other fractions (M50)
M1.1: Breeding suggestions for WP3 based on extractability and protein product characteristics (M12).

D2.1 Report on protein characterization and functional properties from a range of feedstocks: Protein characterization (M 28), Protein functionality (M36)
D2.2 Note on application of RuBisCO in "real" product (M53)
M2.1. Link between protein extraction and protein functionality in specific applications (M42)

D3.1: List of QTLs and candidate genes involved in extractability efficiency (M36)
D3.2: A list of genes or pathways that play a key role in protein functionalities or extractability in by products, to target by breeding. (M48)
M3.1: Field trials of GWAS panel set up in at least one location (M20)

D4.1: Detailed project plan with research questions, tasks and activities, based on priorities from scoping workshop (M6)
D4.2: Communication and dissemination of project results in publications, workshops, presentations and newsletters (M11, M23, M39, M51)
D4.3: Project management including annual reporting (M12, M24, M36, M54).

Naam projectleider

Marieke Bruins