Understanding Haploid Embryo Formation in Plants

Understanding Haploid Embryo Formation in Plants






Landbouw, Water, Voedsel>A. Kringlooplandbouw>A2. Gezonde, robuuste bodem en teeltsystemen gebaseerd op agro-ecologie en zonder schadelijke emissies naar grond- en oppervlaktewater






Doubled-haploid (DH) production is an in vitro propagation technology that is used to facilitate plant breeding by generating true breeding (homozygous) lines in a single generation. DH production relies on in vitro embryo formation from male or female cells of the haploid gametophyte, referred to respectively as androgenesis and gynogenesis, followed by chromosome doubling to produce DH plants. DH technology is widely used to accelerate the plant breeding process, and for the development of true-breeding F1 parents and commercial cultivars. This process is highly genotype dependent and many crops, including tomato, are recalcitrant for DH production.
This project aims to understand the developmental and genetic processes that lead to the formation of an embryo from the unfertilized female gametophyte (parthenogenesis) and to use this information as a framework to obtain gynogenic DH plants in tomato. The project is targeted to professional plant breeding companies. Rijk Zwaan is the sole partner in the project.

Doel van het project

The main goal of this project is to develop novel haploid breeding technologies for tomato, the second largest vegetable crop and a major Dutch greenhouse crop. Haploid breeding is used to accelerate the development of breeding material and commercial lines. Tomato is recalcitrant for haploid production, and currently no efficient systems are available for this crop.

Relatie met missie (Motivatie)

This project contributes to the ST Key Technologies, MMIP Key Technologies 2 (ST2) ‘Biotechnology and Breeding’, Mission 44 ‘Generic Key Technologies’. This proposal will develop ‘innovative (haploid) breeding methods’, one of the sub-themes within this priority. Many crops are recalcitrant for haploid breeding and therefore rely on the slower process of classical breeding for the development of new lines. The generic technology developed in this project will be applicable for haploid production in a wide range of recalcitrant crops.

Geplande acties

The following results are foreseen at the end of the project:
1. A developmental description of the main steps in embryo sac and early embryo and endosperm development in tomato.
2. Identification of chemical enhancers of gynogenesis..
3. Transcription factor-induced parthenogenesis.

Naam projectleider

Kim Boutilier